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The Protein-Disorder Module of BioJava ===================================================== A tutorial for the protein-disorder module of [BioJava](http://www.biojava.org) ## About

The protein-disorder module of BioJava provide an API that allows to predict protein-disorder using the JRONN algorithm

## How can I predict disordered regions on a protein sequence? ----------------------------------------------------------- BioJava provide a module *biojava-protein-disorder* for prediction disordered regions from a protein sequence. Biojava-protein-disorder module for now contains one method for the prediction of disordered regions. This method is based on the Java implementation of [RONN](http://www.strubi.ox.ac.uk/RONN) predictor. This code has been originally developed for use with [JABAWS](http://www.compbio.dundee.ac.uk/jabaws). We call this code *JRONN*. *JRONN* is based on the C implementation of RONN algorithm and uses the same model data, therefore gives the same predictions. JRONN based on RONN version 3.1 which is still current in time of writing (August 2011). Main motivation behind JRONN development was providing an implementation of RONN more suitable to use by the automated analysis pipelines and web services. Robert Esnouf has kindly allowed us to explore the RONN code and share the results with the community. Original version of RONN is described in [Yang,Z.R., Thomson,R., McMeil,P. and Esnouf,R.M. (2005) RONN: the bio-basis function neural network technique applied to the detection of natively disordered regions in proteins. Bioinformatics 21: 3369-3376](http://bioinformatics.oxfordjournals.org/content/21/16/3369.full) Examples of use are provided below. For more information please refer to JronnExample testcases. Finally instead of an API calls you can use a [ command line utility](http://biojava.org/wikis/BioJava:CookBook3:ProteinDisorderCLI/ "wikilink"), which is likely to give you a better performance as it uses multiple threads to perform calculations. Example 1: Calculate the probability of disorder for every residue in the sequence ---------------------------------------------------------------------------------- ```java FastaSequence fsequence = new FastaSequence("name", "LLRGRHLMNGTMIMRPWNFLNDHHFPKFFPHLIEQQAIWLADWWRKKHC" + "RPLPTRAPTMDQWDHFALIQKHWTANLWFLTFPFNDKWGWIWFLKDWTPGSADQAQRACTWFFCHGHDTN"); float[] rawProbabilityScores = Jronn.getDisorderScores(fsequence); ``` Example 2: Calculate the probability of disorder for every residue in the sequence for all proteins from the FASTA input file ----------------------------------------------------------------------------------------------------------------------------- ```java final List sequences = SequenceUtil.readFasta(new FileInputStream("src/test/resources/fasta.in")); Map rawProbabilityScores = Jronn.getDisorderScores(sequences); ``` Example 3: Get the disordered regions of the protein for a single protein sequence ---------------------------------------------------------------------------------- ```java FastaSequence fsequence = new FastaSequence("Prot1", "LLRGRHLMNGTMIMRPWNFLNDHHFPKFFPHLIEQQAIWLADWWRKKHC" + "RPLPTRAPTMDQWDHFALIQKHWTANLWFLTFPFNDKWGWIWFLKDWTPGSADQAQRACTWFFCHGHDTN"+ "CQIIFEGRNAPERADPMWTGGLNKHIIARGHFFQSNKFHFLERKFCEMAEIERPNFTCRTLDCQKFPWDDP"); Range[] ranges = Jronn.getDisorder(fsequence); ``` Example 4: Calculate the disordered regions for the proteins from FASTA file ---------------------------------------------------------------------------- ```java final List sequences = SequenceUtil.readFasta(new FileInputStream("src/test/resources/fasta.in")); Map ranges = Jronn.getDisorder(sequences); ``` ## License The content of this tutorial is available under the [CC-BY](http://creativecommons.org/licenses/by/3.0/) license, available [here](../license.md). ## Please Cite **BioJava 5: A community driven open-source bioinformatics library**
*Aleix Lafita, Spencer Bliven, Andreas Prli, Dmytro Guzenko, Peter W. Rose, Anthony Bradley, Paolo Pavan, Douglas Myers-Turnbull, Yana Valasatava, Michael Heuer, Matt Larson, Stephen K. Burley, & Jose M. Duarte*
[PLOS Computational Biology (2019) 15 (2):e1006791.](https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1006791)
[](https://doi.org/10.1371/journal.pcbi.1006791) [](http://www.ncbi.nlm.nih.gov/pubmed/30735498) --- Navigation: [Home](../README.md) | Book 3: The Protein Structure modules Prev: [Book 4: The Genomics Module](../genomics/README.md) | Next: [Book 6: The ModFinder Module](../modfinder/README.md)

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