This repository was archived by the owner on Oct 17, 2022. It is now read-only.
This repository was archived by the owner on Oct 17, 2022. It is now read-only.
Description
Hi SmithLab team!
I downloaded Test data, at http://smithlabresearch.org/software/methpipe/
After installed methpipe, I tested with downloaded data but had problems when running commands, such as methcounts; pmd; etc
1. methcounts
methcounts -o $rs/binh1.meth -c $gen/ -v $rs/snippet_uniq.mr
n_chrom_files: 1
n_chroms: 1
bad line in MappedRead file: chr22 29971 30072 FREDDYKRUEGER_0001:1:26:268:1868#0/2 0 - AGGTTTAAGGTTTTTAAAATAGTTGATATTTTTAAATTGGTTTTTTTGTATATTAAATTAAAGAAATTAGGTTTTTAAATTAAGGAAAATGAATGATAGTTe_ab_adg_ggggagec]c]_ab^bageggggggggggbggggfgggggegbggggegfgggggffegfggggggggebefdgggggeegggggffebggg
2. pmd
pmd -o Human_ColonCancer-chr19.pmd -v -i 15 Human_ColonCancer-chr19.meth
[DYNAMICALLY SELECTING BIN SIZE]
evaluating bin size 1000
ERROR: bad methpipe site line: "chr19 60118 60119 CpG:0 0 +"
MacOS Catalina, 10.15.6.
Terminal: zsh shell, 5.8 (x86_64-apple-darwin19.3.0)
Please, help me to resolve those issues. Thank you!
Reactions are currently unavailable
Hi SmithLab team!
I downloaded Test data, at http://smithlabresearch.org/software/methpipe/
After installed methpipe, I tested with downloaded data but had problems when running commands, such as methcounts; pmd; etc
1. methcounts
methcounts -o $rs/binh1.meth -c $gen/ -v $rs/snippet_uniq.mr
n_chrom_files: 1
n_chroms: 1
bad line in MappedRead file: chr22 29971 30072 FREDDYKRUEGER_0001:1:26:268:1868#0/2 0 - AGGTTTAAGGTTTTTAAAATAGTTGATATTTTTAAATTGGTTTTTTTGTATATTAAATTAAAGAAATTAGGTTTTTAAATTAAGGAAAATGAATGATAGTTe_ab_adg_ggggagec]c]_ab^bageggggggggggbggggfgggggegbggggegfgggggffegfggggggggebefdgggggeegggggffebggg
2. pmd
pmd -o Human_ColonCancer-chr19.pmd -v -i 15 Human_ColonCancer-chr19.meth
[DYNAMICALLY SELECTING BIN SIZE]
evaluating bin size 1000
ERROR: bad methpipe site line: "chr19 60118 60119 CpG:0 0 +"
MacOS Catalina, 10.15.6.
Terminal: zsh shell, 5.8 (x86_64-apple-darwin19.3.0)
Please, help me to resolve those issues. Thank you!